Lane 1: 2-log ladder (0.10-10Kb) from NEB
Lane 2 - KZ: touchdown PCR reaction for isolate MG030416_10 (PCR code: KZ13) with Forward Primer MG2208 and Reverse primer MG2214. No results appeared for this lane and subsequent colonies did not grow when this product was used for a Site Directed Mutageneis KLD reaction and E. coli transformation.
Lane 3 - KZ: Digestion reaction for pDD 286 RFP N-terminal fusion with restriction enzymes ClaI and NgoMIV (Digest code: KZ11). Bands appear around the 6500bp, 2600bp and 610bp regions. These bands appear as expected.
Lane 5 - AH11: PCR amplification for unc-11 C-Terminal fusion, project 5. Template ID: 040316-10. Performed PCR amplification with 2 primers: MG2203_sgRNA_R_6141, MG2212_Unc-11_C. . No PCR bands seen for product but achieved successful transformation.
Lane 6 - SC
Lane 7 - SC
Lane 2 - touchdown PCR reaction for isolate MG030416_10 (PCR code: KZ 13) with Forward Primer 2208 and Reverse primer 2214. No results appeared for this lane and subsequent colonies did not grow when this product was used for a Site Directed Mutageneis KLD reaction and E. coli transformation.
ReplyDeleteLane 3 - Digestion reaction for pDD 286 RFP N-terminal fusion with restriction enzymes ClaI and NgoMIV (Digest code: KZ11). Bands appear around the 6500bp, 2600bp and 610bp regions. These bands appear as expected.